Interactive installation for museum, Move or Die: Influence of embodied interaction on players’ experience.

This thesis presents the interactive game installation, Move or Die, created for an exhibit at The Finnish Museum of Natural History. It documents the development of the project by describing ideas behind the concept, detailed components of the installation, and reasons for the design decisions made during the production process. For the theoretical section, the thesis investigates the influence of physical interaction on the players’ experience based on the embodied approach. The museum context is considered a great deal in both the production and theory sections. Move or Die is a gamified installation in which participants can save endangered species through bodily interaction. As the 'game' is projected onto the floor and Kinect sensors installed on the ceiling register movement, participants can catch and move the graphic animals on the floor by using their arms. Move or Die reflects a research method of the museum called 'assisted migration' and contains their research contents. It is designed to inform people of the situation of the endangered species and to offer participatory experience to the visitors at the same time. It is the objective of Move or Die to simulate the experience of saving endangered species with the player’s own intention and physical ability. Therefore, to investigate the interaction of Move or Die, it is significant to understand the players’ experience. To analyze this interaction, three representa- tive aspects of the interaction are discussed separately: full- body interaction, social interaction, and reality-based interaction. This thesis tries to uncover comprehensive influences of the interaction beyond its basic functionality

A Fully-Human Antibody Specifically Targeting a Membrane-Bound Fragment of CADM1 Potentiates the T Cell-Mediated Death of Human Small-Cell Lung Cancer Cells

Small-cell lung cancer (SCLC) is the most aggressive form of lung cancer and the leading cause of global cancer-related mortality. Despite the earlier identification of membrane-proximal cleavage of cell adhesion molecule 1 (CADM1) in cancers, the role of the membrane-bound fragment of CAMD1 (MF-CADM1) is yet to be clearly identified. In this study, we first isolated MF-CADM1-specific fully human single-chain variable fragments (scFvs) from the human synthetic scFv antibody library using the phage display technology. Following the selected scFv conversion to human immunoglobulin G1 (IgG1) scFv-Fc antibodies (K103.1–4), multiple characterization studies, including antibody cross-species reactivity, purity, production yield, and binding affinity, were verified. Finally, via intensive in vitro efficacy and toxicity evaluation studies, we identified K103.3 as a lead antibody that potently promotes the death of human SCLC cell lines, including NCI-H69, NCI-H146, and NCI-H187, by activated Jurkat T cells without severe endothelial toxicity. Taken together, these findings suggest that antibody-based targeting of MF-CADM1 may be an effective strategy to potentiate T cell-mediated SCLC death, and MF-CADM1 may be a novel potential therapeutic target in SCLC for antibody therapy